Phospho smad3

Author: kelx

August undefined, 2024

WebThe SMAD3 gene provides instructions for making a protein involved in transmitting chemical signals from the cell surface to the nucleus. This signaling pathway, called the transforming growth factor-beta (TGF-β) pathway, allows the environment outside the cell to affect cell function, including how the cell produces other proteins.

Macrophage Smad3 Protects the Infarcted Heart ... - Circulation …

WebAlthough the upregulation of Smad3 in VSMCs had no significant effect on total p27 levels, Smad3 did stimulate the phosphorylation of p27 at serine-10 as well as the nuclear export of p27, events associated with cell proliferation. WebEndogenous Smad3 and phospho-β-catenin were probed. (F) HA-Smad3 was overexpressed in 293T cells with vector or GSK3-β mutants (S9A or Y216F). After MG-132 treatment (30 μM, 3 h), cells were harvested in RIPA buffer for the Smad3 ubiquitination assay. We next undertook three different ways to perturb the function of endogenous GSK3-β in ... floral farms boston

SMAD3 phospho T179 Antibody (600-401-C48) Rockland

WebMay 16, 2024 · Smad3-dependent phagocytosis is mediated, at least in part, through induction of Mfge8 (milk fat globule-epidermal growth factor factor 8), whereas Smad3-mediated PPAR (peroxisome proliferator-activated receptor) upregulation may be involved in acquisition of anti-inflammatory properties. Methods WebBoster Bio Anti-Phospho-SMAD3 S423/S425 Antibody catalog # P00059. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μL in Liquid … WebMar 13, 2024 · Taken together with the elevated phospho-Smad3 levels in T2D islets (Fig. 7a, b), these findings are consistent with an autocrine role for TGF-β1 in β-cell apoptosis. floral farms neighborhood plan

Anti-phospho-Smad3 (Ser423/425) Antibody 07-1389

WebAnti-phospho-Smad2/3 (pThr8) antibody produced in rabbit (affinity isolated antibody); Suitable for ELISA; Anti-phospho-Smad3 (pSer425) antibody has been used in western blotting; SMAD3 (homologues of the Drosophila protein, mothers against decapentaplegic (Mad) and the Caenorhabditis ele WebPurity/Specificity. Anti-SMAD3 pT179 affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pT179 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. great scott expressionWebDec 30, 2024 · Phosphorylated forms (phospho-isoforms) in Smad-related pathways can transmit opposing signals: cytostatic C-terminally-phosphorylated Smad3 (pSmad3C) and … great scott expression origin

"WebSMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. " - Phospho smad3

Phospho smad3

Protection from β-cell apoptosis by inhibition of TGF-β/Smad3 …

WebSmad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by … WebPhospho-Smad3 (S423/S425)/Smad2 (S465/S467) Cell-Based ELISA Summary. Alternatively. * Alternatively, this assay can be run over two days, which requires 2 hours …

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WebThis antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested. Purity/Specificity This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. WebThe Phospho-SMAD3 (Ser423/425) assay measures SMAD3 when phosphorylated at Ser423/425. Contrary to Western Blot, the assay is entirely plate-based and does not …

WebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health Analytical & Enterprise Solutions An Analytical and Enterprise solutions business, including leading OneSource laboratory services, focused on accelerating scientific outcomes WebJul 1, 2012 · Future studies will elucidate the impact of phospho-specific Smad3 signaling in the context of other TGFβ family members, including activin and Nodal, as well as other kinases and cancer models. Additionally, determining the differential function of Smad2 and Smad3 may be key to understanding the role of Smad signaling during carcinogenesis ...

WebDescription Rabbit polyclonal to Smad2 + Smad3 (phospho T8) Host species Rabbit Tested applications Suitable for: WB more details Species reactivity Reacts with: Mouse, Human Predicted to work with: Rat Immunogen Synthetic peptide corresponding to Human Smad2 + Smad3 (phospho T8). WebApr 7, 2024 · Moreover, deletion of Smad3 from db/m or db/db mice almost competitively blocked SARS-CoV-2 N-induced Ripk3/MLKL expression and phospho-MLKL level (Fig. …

WebPhospho-Smad3(Ser423/425) 磷酸化细胞信号转导分子SMAD3抗体 0.1ml Phospho-Smad3(Ser425) 磷酸化细胞信号转导分子SMAD3抗体 0.1ml Phospho-Smad3(Ser208) 磷酸化细胞信号转导分子SMAD3抗体 0.1ml SMC1 染色体结构维持蛋白1抗体 0.2ml 人抗血小板抗体IgG(PA-IgG)ELISA试剂盒Phospho-SMC1 (Ser360) 磷酸化 ...

WebMay 5, 2024 · p-Smad3 Antibody (1D9) is an IgG mouse monoclonal p-Smad3 antibody suitable for the detection of Ser 425 phosphorylated Smad3 of mouse, rat and human … floral farms humboldt countyWebSMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. … great scott family dinerWebSpecificity ab51451 is specific for the phosphorylated form of the protein although minimal reactivity with nonphosphorylated human Smad3 can be observed by ELISA and WB. ab51451 also reacts with phosphorylated Smad1 and Smad5 but negligible reactivity is seen against other phosphorylated Smad family members. Tested applications floral fascinator derby louisville floristWebSep 30, 1997 · A Smad3 protein in which the three C-terminal serines have been replaced by aspartic acids is also a dominant inhibitor of TGF-β signaling, but can activate plasminogen activator inhibitor 1 (PAI-1) transcription in a ligand-independent fashion when its nuclear localization is forced by transient overexpression. floral fascination plateWebAug 20, 2024 · (A) Representative western blot using the indicated antibodies. (B) Quantitative analysis of Phospho-Smad2 and Phospho-Smad3 protein levels of five independent experiments. (C,D) T98G cells were transiently transfected with 2 μg of Smad3 and/or Smad4 expression vectors and cultured for 24 h in serum-deprived medium. floral fashions port chester new yorkPhospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb detects endogenous levels of Smad3 when phosphorylated at Ser423/425. This antibody does not cross-react with other family members. Species Reactivity: Human, Mouse, Rat. Species predicted to react based on 100% sequence homology: great scott family diner westbrookWebab52903 detects Smad3 phosphorylated on Serine 423 and Serine 425. This Smad3 antibody may also detect Smad1, Smad2 and Smad5 phosphorylated at the equivalent sites. Tested applications Suitable for: … floral feast african honey