Ime intron plant

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August undefined, 2024

Witryna14 lip 2016 · IME by the GGT1 5′UTR intron was at least 2-fold. Chromatin immunoprecipitation experiments showed that the abundance of RNA polymerase II binding to the intron-less construct is reduced. ... The analysis of gusA unspliced RNA accumulation in GGT1 Ɗ5I::gusA plants revealed that IME already takes place on … WitrynaIntrons of plant tubulin genes are important regulatory elements for supporting gene expression. Introns can either increase the level of expression through intron-mediated enhancement (IME), or they can change the actual site of gene expression. The molecular basis of both IME and intron dependent spatial expression (IDSE) remains …

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Witryna12 sie 2024 · Here we demonstrated that intron-mediated enhancement (IME) is a suitable tool to boost circRtn4 formation leading to significant levels of translation, compared to very low or even lack of ... fit htl leonding

In vivo measurements reveal a single 5′-intron is sufficient …

WitrynaIntrons of plant tubulin genes are useful molecular tools to study IME (Intron Mediated Enhancement of gene expression) and to define plant genetic and evolutionary … WitrynaIME; however, a large internal deletion of the first intron of the maize Adh gene reduces splicing and con sequently IME (Luehrsen and Walbot 1994b). A point mutation at the 5'- or 3'- splice site of the maize Hsp82 intron impaired splicing, suggesting that IME requires intron splicing (Sinibaldi and Mettler 1992). In contrast, Witryna5'UTR IME in plants, especially those within the intron itself, remain poorly defined. The plant Arabidopsis thaliana has a compact genome and generally small introns [18], consistent with the ... fiththird.com

Transcriptional and post-transcriptional enhancement of gene

Testing the IMEter on rice introns and other aspects of intron …

WitrynaHowever, many introns that affect expression increase mRNA accumulation through an unknown mechanism, referred to as intron-mediated enhancement (IME). Despite … Witryna12 mar 2024 · ZmbZIP25 (Zea mays bZIP (basic leucine zipper) transcription factor 25) is a function-unknown protein that belongs to the D group of the bZIP transcription factor family. RNA-seq data showed that the expression of ZmbZIP25 was tissue-specific in maize silks, and this specificity was confirmed by RT-PCR (reverse transcription … fith syndromeWitryna5 sie 2016 · Key message Related motifs strongly increase gene expression when added to an intron located in coding sequences. Abstract Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific … fith thrid bandk

"Witryna1 cze 2008 · Introns mediate complex gene regulation via alternative splicing; they may act in cis as expression enhancers through IME (intron-mediated enhancement of gene expression) and in trans as negative regulators through the generation of intronic microRNA. Furthermore, some introns also contain promoter sequences for … " - Ime intron plant

Ime intron plant

The enduring mystery of intron-mediated enhancement

Witryna24 cze 2024 · Consistent with plant and mammalian studies, we determined a single, natural or synthetic, 5′-intron is sufficient for the full IME effect conferred by three … Witryna6 sty 2024 · The list summarizes information on the given publications in the following way: (i) intron in combination with specific promoters (E – endogenous promoter …

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Witryna22 paź 2024 · Plant biotechnology traits provide a means to increase crop yields, manage weeds and pests, and sustainably contribute to addressing the needs of a growing population. One of the key challenges in developing new traits for plant biotechnology is the availability of expression elements for efficacious and predictable … Witryna26 maj 2024 · Background: Intron mediated enhancement (IME) is the potential of introns to enhance the expression of its respective gene. This essential function of …

Witryna2 dni temu · Introduction. Recent studies showed that alternative splicing (AS) is highly pervasive in plants (Reddy et al., 2013), and introns of more than 60% genes undergo this process (Syed et al., 2012).AS plays a key role in the regulation of gene regulatory networks to help plants adapt to environmental fluctuations (Syed et al., … Witryna1 paź 2024 · In many eukaryotes, including mammals, plants, yeast, and insects, introns can increase gene expression without functioning as a binding site for transcription factors. This phenomenon was termed ‘intron-mediated enhancement’. ... The role of splicing in IME and the significance of intron position relative to the sites of …

WitrynaIntrons of plant tubulin genes are important regulatory elements for supporting gene expression. Introns can either increase the level of expression through intron … WitrynaIntrons of plant tubulin genes are useful molecular tools to study IME (Intron Mediated Enhancement of gene expression) and to define plant genetic and evolutionary relationships through ILP (Intron Length Polymorphism). Here we show that the intron present within the 5′UTR sequence of some rice β-tubulin genes can sustain IME in …

Witryna1 sie 2015 · The term intron mediated enhancement (IME) has been used as an umbrella to include any case in which an intron-containing construct is expressed …

Witryna19 wrz 2024 · UDP-glycosyltransferase (UGT) plays an essential role in regulating the synthesis of hormones and secondary metabolites in plants. In this study, 129 members of the Petunia UGT family were identified and classified into 16 groups (A–P) based on phylogenetic analysis. The same subgroups have conserved motif … can honey be used on the faceWitryna22 cze 2024 · Intron-mediated enhancement (IME) is a well-known phenomenon to enhance homogeneous protein expression in plants and animals (Vain et al., 1996; … fit hub altiraWitryna28 kwi 2008 · However, the mechanism underlying intron-mediated enhancement (IME) in plants is largely unknown. Rose et al. (pages 543–551) adopted a bioinformatics … fithub arteriWitryna1 maj 2008 · Introns of plant tubulin genes are useful molecular tools to study IME (Intron Mediated Enhancement of gene expression) and to define plant genetic and … can honey conduct electricityWitrynaThis process of intron-mediated enhancement (IME) is most thoroughly studied in Arabidopsis thaliana, where it has been shown that enhancing introns are typically located near the promoter and are compositionally distinct from downstream introns. In this study, we perform a comprehensive comparative analysis of several sequenced … can honey be used to sweeten coffeeWitryna19 maj 2006 · However, the intrinsic determinants of 5'UTR IME in plants, especially the role of any sequence motifs within the intron, remain poorly defined. ... Luehrsen K, Walbot V: Addition of A- and U-rich sequence increases the splicing efficiency of a deleted form of a maize intron. Plant Mol Biol. 1994, 24: 449-463. … can honey change your eye colorWitrynaThis has been described as “intron-mediated enhancement” or IME (Gallegos and Rose, 2015), although the need for a more specific name is becoming increasingly apparent as new information emerges, and to avoid confusion with the general use of the same phrase to describe any boost in expression caused by an intron regardless of … can honey cause indigestion