WebMar 11, 2016 · To determine whether the increase in phosphorylation of AMPK at Ser 485/491 was attributable to PKC activation, we used the non-selective PKC inhibitor Gö6983. Treatment of C2C12 myotubes with Gö6983 (5 μ m) for 1 h prior to PMA treatment (50 n m, 30 min) significantly attenuated phosphorylation of AMPK at Ser 485/491 (Fig. … WebSep 1, 2024 · As shown in Fig. 1 C and D, IR C2C12 cells exhibited impaired glucose uptake capacity as well as decreased expression levels of GLUT4 and insulin receptor. Moreover, western blot analysis showed that the phosphorylation of the insulin-related signaling pathways AKT, p38 and ERK was impaired in IR C2C12 cells (Fig. 1 D).
C2C12 cell model: its role in understanding of insulin resistance at ...
WebC2C12 grow as undifferentiated myoblasts in growth medium, GM [DMEM—high glucose no sodium pyruvate (Gibco), 10–20% FBS (HyClone or Gibco), 1% penicillin/streptomycin, … WebSep 13, 2024 · Irisin increased ERK but not p38 phosphorylation in a dose-dependent manner in C2C12 cells . Irisin increased ERK phosphorylation levels the most at 20 min after treatment, while p38 … kwak dong-yeon dramas list
Leucine modulates dynamic phosphorylation events in insulin …
WebFeb 5, 2024 · TGFβ stimulates MEK phosphorylation. C2C12 cells were seeded onto cell culture plates at equal density and maintained in TGFβ (1ng/mL), CT-1 (10ng/mL), or solvent. Total protein samples were extracted from the cells and equal amounts of total protein (20 μg) were subjected to Western blotting analysis. WebMar 20, 2014 · Background Branched-chain amino acids, especially leucine, are known to interact with insulin signaling pathway and glucose metabolism. However, the mechanism by which this is exerted, remain to be clearly defined. In order to examine the effect of leucine on muscle insulin signaling, a set of experiments was carried out to quantitate … WebMar 6, 2024 · insulin sensitivity was found to be higher in mouse C2C12 than rat L6 cells.[21] Apart from differences in insulin sensi-tivity, mouse C2C12 and rat L6 cell lines presented different responsiveness to muscle wasting.[24] When compared to rat L6 cells, mouse C2C12 cells possess genes that are asso-ciated with muscle development and … jazzeppi\u0027s restaurant